Journal: Cancer biology & therapy

Article Title: The radiation-induced cell-death signaling pathway is activated by concurrent use of cisplatin in sequential biopsy specimens from patients with cervical cancer.

doi: 10.4161/cbt.6.6.4098

Figure Lengend Snippet: Figure 2. Expression values of four CRT-responsive genes evaluated by microarray in pretreatment (pre) and midtreatment (mid) samples. Normalized expression values are shown for CDKN1A, BAX, TNFSF8 and RRM2B in 19 CRT cases and 20 RT cases. The open circle indicates a patient with a p53 mutation. Significant upregu‑ lation of these genes was induced by CRT. RT-treated samples showed variation between patients in the change of expression of these genes.

Article Snippet: They were stained with Cy5‐streptavidin (Amersham Biosciences) and scanned using a dual‐laser microarray scanner (Agilent).

Techniques: Expressing, Microarray, Mutagenesis

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Tumor immune escape mechanisms: impact of the neuroendocrine system

doi: 10.1007/s00262-006-0126-x

Figure Lengend Snippet: Tissue microarrays of normal tissues and tumors of the respective tissue. First and third lane: normal tissue; second and fourth lane: tumors of the same type of tissue as the normal tissue above. Microarrays containing frozen sections of various tissue samples were purchased from BioCat (Heidelberg, Germany). In brief, the sections were treated with 0.3% H2O2 for 30 min at room temperature (RT). After washing with PBS, the sections were blocked with 10% normal bovine serum for 1 h at RT and subsequently stained with a rabbit anti-human-β2-adrenoceptor antibody (a Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse anti-human neurofilament protein antibody (b Dako Cytomatation, Hamburg, Germany) at 4°C overnight. After washing with PBS, the arrays were incubated with a Cy3-coupled secondary goat anti-rabbit or anti-mouse antibody (Dianova, Hamburg, Germany) for 1 h at RT, and the fluorescence of the samples was analyzed using a dual-laser microarray scanner. In (c), the respective tissue origin is shown

Article Snippet: After washing with PBS, the arrays were incubated with a Cy3-coupled secondary goat anti-rabbit or anti-mouse antibody (Dianova, Hamburg, Germany) for 1 h at RT, and the fluorescence of the samples was analyzed using a dual-laser microarray scanner.

Techniques: Staining, Incubation, Fluorescence, Microarray